Cancer Cell-specific Transfection of hCas9 Gene Using Ad5F35 Vector

Background: The clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas9) is thought to have promising clinical potential. However, the off-target effects of Cas9 are a major concern for its application. Therefore, we hypothesized that adverse gene editing might be minimized if human codon-optimized Streptococcus pyogenes (hCas9) could specifically expressed in cancer cells. Materials and Methods: We constructed chimeric adenoviral vector, Ad5F35-MKp-hCas9, infected bladder cell lines with this vector. confirmation hCas9 expression was performed 3-4 days after from infection. Results: observed Ad5F35-MKp-hCas9 cells but not non-malignant Conclusion: Our study showed vector capable expressing high specificity These findings may help minimizing risk editing.